pci sep glua2 Search Results


sep glua2 r  (Addgene inc)
93
Addgene inc sep glua2 r
Sep Glua2 R, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sep glua2 r/product/Addgene inc
Average 93 stars, based on 1 article reviews
sep glua2 r - by Bioz Stars, 2026-03
93/100 stars
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plasmid pci sep glur2 q  (Addgene inc)
92
Addgene inc plasmid pci sep glur2 q
a , Schematic depicting AMPAR trafficking downstream of BDNF–TrkB–CAMKII signalling . b , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI glioma with or without BDNF treatment for 5, 15 and 30 min. c , Quantification of cell surface GluA4 in b ( n = 3 independent biological replicates). d , Western blot analysis of cell surface and total cell protein levels of GluA3 in SU-DIPG-VI glioma with or without BDNF treatment for 30 min. e , Quantification of cell surface GluA3 in d ( n = 3 independent biological replicates). f , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI cells treated with NLGN3 for 30 min. g , Quantification of cell surface GluA4 data in f ( n = 3 independent biological replicates). h , Schematic showing <t>GluA2–SEP</t> experiments. i , j , Validation of pHluorin approach. i , Left, representative images of a glioma cell process expressing <t>GluA2(Q)–SEP,</t> PSD95–RFP and whole-cell TagBFP in co-culture with neurons. Right, representative GluA2(Q)–SEP puncta. Scale bars, 5 µm (left) and 1 µm (right). Cells were exposed to pH 7.4 followed by pH 5.5 and then pH 7.4. j , Quantification of fluorescence intensity of GluA2(Q)–SEP puncta before, during and after acidic exposure ( n = 4 puncta from a representative cell). k , Top, representative images of two processes from glioma cells expressing GluA2(Q)–SEP, PSD95–RFP and TAG-BFP2 in co-culture with neurons (scale bar, 5 µm). Middle and bottom, representative images of GluA2(Q)–SEP puncta at 0, 5, 15 and 20 min of BDNF incubation (scale bar=1 µm). l , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta over time with BDNF treatment ( n = 8 puncta, 6 cells). m , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta after 15 min versus basal fluorescence in control (vehicle, n = 4 puncta, 2 cells) or BDNF-treated cells ( n = 8 puncta, 6 cells). Data are mean ± s.e.m. Two-tailed unpaired Student’s t -test ( c , e , g , m ); two-tailed paired Student’s t -test ( j ); two-tailed one-sample t -test ( l ).
Plasmid Pci Sep Glur2 Q, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid pci sep glur2 q/product/Addgene inc
Average 92 stars, based on 1 article reviews
plasmid pci sep glur2 q - by Bioz Stars, 2026-03
92/100 stars
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plasmid pci-sep-glur2(q)  (Addgene inc)
90
Addgene inc plasmid pci-sep-glur2(q)
a , Schematic depicting AMPAR trafficking downstream of BDNF–TrkB–CAMKII signalling . b , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI glioma with or without BDNF treatment for 5, 15 and 30 min. c , Quantification of cell surface GluA4 in b ( n = 3 independent biological replicates). d , Western blot analysis of cell surface and total cell protein levels of GluA3 in SU-DIPG-VI glioma with or without BDNF treatment for 30 min. e , Quantification of cell surface GluA3 in d ( n = 3 independent biological replicates). f , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI cells treated with NLGN3 for 30 min. g , Quantification of cell surface GluA4 data in f ( n = 3 independent biological replicates). h , Schematic showing <t>GluA2–SEP</t> experiments. i , j , Validation of pHluorin approach. i , Left, representative images of a glioma cell process expressing <t>GluA2(Q)–SEP,</t> PSD95–RFP and whole-cell TagBFP in co-culture with neurons. Right, representative GluA2(Q)–SEP puncta. Scale bars, 5 µm (left) and 1 µm (right). Cells were exposed to pH 7.4 followed by pH 5.5 and then pH 7.4. j , Quantification of fluorescence intensity of GluA2(Q)–SEP puncta before, during and after acidic exposure ( n = 4 puncta from a representative cell). k , Top, representative images of two processes from glioma cells expressing GluA2(Q)–SEP, PSD95–RFP and TAG-BFP2 in co-culture with neurons (scale bar, 5 µm). Middle and bottom, representative images of GluA2(Q)–SEP puncta at 0, 5, 15 and 20 min of BDNF incubation (scale bar=1 µm). l , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta over time with BDNF treatment ( n = 8 puncta, 6 cells). m , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta after 15 min versus basal fluorescence in control (vehicle, n = 4 puncta, 2 cells) or BDNF-treated cells ( n = 8 puncta, 6 cells). Data are mean ± s.e.m. Two-tailed unpaired Student’s t -test ( c , e , g , m ); two-tailed paired Student’s t -test ( j ); two-tailed one-sample t -test ( l ).
Plasmid Pci Sep Glur2(Q), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid pci-sep-glur2(q)/product/Addgene inc
Average 90 stars, based on 1 article reviews
plasmid pci-sep-glur2(q) - by Bioz Stars, 2026-03
90/100 stars
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pci-sep-glur2  (Promega)
90
Promega pci-sep-glur2
a , Schematic depicting AMPAR trafficking downstream of BDNF–TrkB–CAMKII signalling . b , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI glioma with or without BDNF treatment for 5, 15 and 30 min. c , Quantification of cell surface GluA4 in b ( n = 3 independent biological replicates). d , Western blot analysis of cell surface and total cell protein levels of GluA3 in SU-DIPG-VI glioma with or without BDNF treatment for 30 min. e , Quantification of cell surface GluA3 in d ( n = 3 independent biological replicates). f , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI cells treated with NLGN3 for 30 min. g , Quantification of cell surface GluA4 data in f ( n = 3 independent biological replicates). h , Schematic showing <t>GluA2–SEP</t> experiments. i , j , Validation of pHluorin approach. i , Left, representative images of a glioma cell process expressing <t>GluA2(Q)–SEP,</t> PSD95–RFP and whole-cell TagBFP in co-culture with neurons. Right, representative GluA2(Q)–SEP puncta. Scale bars, 5 µm (left) and 1 µm (right). Cells were exposed to pH 7.4 followed by pH 5.5 and then pH 7.4. j , Quantification of fluorescence intensity of GluA2(Q)–SEP puncta before, during and after acidic exposure ( n = 4 puncta from a representative cell). k , Top, representative images of two processes from glioma cells expressing GluA2(Q)–SEP, PSD95–RFP and TAG-BFP2 in co-culture with neurons (scale bar, 5 µm). Middle and bottom, representative images of GluA2(Q)–SEP puncta at 0, 5, 15 and 20 min of BDNF incubation (scale bar=1 µm). l , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta over time with BDNF treatment ( n = 8 puncta, 6 cells). m , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta after 15 min versus basal fluorescence in control (vehicle, n = 4 puncta, 2 cells) or BDNF-treated cells ( n = 8 puncta, 6 cells). Data are mean ± s.e.m. Two-tailed unpaired Student’s t -test ( c , e , g , m ); two-tailed paired Student’s t -test ( j ); two-tailed one-sample t -test ( l ).
Pci Sep Glur2, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pci-sep-glur2/product/Promega
Average 90 stars, based on 1 article reviews
pci-sep-glur2 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


a , Schematic depicting AMPAR trafficking downstream of BDNF–TrkB–CAMKII signalling . b , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI glioma with or without BDNF treatment for 5, 15 and 30 min. c , Quantification of cell surface GluA4 in b ( n = 3 independent biological replicates). d , Western blot analysis of cell surface and total cell protein levels of GluA3 in SU-DIPG-VI glioma with or without BDNF treatment for 30 min. e , Quantification of cell surface GluA3 in d ( n = 3 independent biological replicates). f , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI cells treated with NLGN3 for 30 min. g , Quantification of cell surface GluA4 data in f ( n = 3 independent biological replicates). h , Schematic showing GluA2–SEP experiments. i , j , Validation of pHluorin approach. i , Left, representative images of a glioma cell process expressing GluA2(Q)–SEP, PSD95–RFP and whole-cell TagBFP in co-culture with neurons. Right, representative GluA2(Q)–SEP puncta. Scale bars, 5 µm (left) and 1 µm (right). Cells were exposed to pH 7.4 followed by pH 5.5 and then pH 7.4. j , Quantification of fluorescence intensity of GluA2(Q)–SEP puncta before, during and after acidic exposure ( n = 4 puncta from a representative cell). k , Top, representative images of two processes from glioma cells expressing GluA2(Q)–SEP, PSD95–RFP and TAG-BFP2 in co-culture with neurons (scale bar, 5 µm). Middle and bottom, representative images of GluA2(Q)–SEP puncta at 0, 5, 15 and 20 min of BDNF incubation (scale bar=1 µm). l , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta over time with BDNF treatment ( n = 8 puncta, 6 cells). m , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta after 15 min versus basal fluorescence in control (vehicle, n = 4 puncta, 2 cells) or BDNF-treated cells ( n = 8 puncta, 6 cells). Data are mean ± s.e.m. Two-tailed unpaired Student’s t -test ( c , e , g , m ); two-tailed paired Student’s t -test ( j ); two-tailed one-sample t -test ( l ).

Journal: Nature

Article Title: Glioma synapses recruit mechanisms of adaptive plasticity

doi: 10.1038/s41586-023-06678-1

Figure Lengend Snippet: a , Schematic depicting AMPAR trafficking downstream of BDNF–TrkB–CAMKII signalling . b , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI glioma with or without BDNF treatment for 5, 15 and 30 min. c , Quantification of cell surface GluA4 in b ( n = 3 independent biological replicates). d , Western blot analysis of cell surface and total cell protein levels of GluA3 in SU-DIPG-VI glioma with or without BDNF treatment for 30 min. e , Quantification of cell surface GluA3 in d ( n = 3 independent biological replicates). f , Western blot analysis of cell surface and total cell protein levels of GluA4 in SU-DIPG-VI cells treated with NLGN3 for 30 min. g , Quantification of cell surface GluA4 data in f ( n = 3 independent biological replicates). h , Schematic showing GluA2–SEP experiments. i , j , Validation of pHluorin approach. i , Left, representative images of a glioma cell process expressing GluA2(Q)–SEP, PSD95–RFP and whole-cell TagBFP in co-culture with neurons. Right, representative GluA2(Q)–SEP puncta. Scale bars, 5 µm (left) and 1 µm (right). Cells were exposed to pH 7.4 followed by pH 5.5 and then pH 7.4. j , Quantification of fluorescence intensity of GluA2(Q)–SEP puncta before, during and after acidic exposure ( n = 4 puncta from a representative cell). k , Top, representative images of two processes from glioma cells expressing GluA2(Q)–SEP, PSD95–RFP and TAG-BFP2 in co-culture with neurons (scale bar, 5 µm). Middle and bottom, representative images of GluA2(Q)–SEP puncta at 0, 5, 15 and 20 min of BDNF incubation (scale bar=1 µm). l , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta over time with BDNF treatment ( n = 8 puncta, 6 cells). m , Fluorescence intensity of co-localized GluA2(Q)–SEP:PSD95–RFP puncta after 15 min versus basal fluorescence in control (vehicle, n = 4 puncta, 2 cells) or BDNF-treated cells ( n = 8 puncta, 6 cells). Data are mean ± s.e.m. Two-tailed unpaired Student’s t -test ( c , e , g , m ); two-tailed paired Student’s t -test ( j ); two-tailed one-sample t -test ( l ).

Article Snippet: The SEP fragment with Gibson overhangs was amplified from Addgene plasmid pCI-SEP-GluR2(Q) (#24002) with primers: 5′-AACGGGTTTGCCGCCAGAACACAGGACCGGTGCCACCATGCAAAAGATTATGCATATTTC-3′ and 5′-CCCCCTATCTGTATGCTGTTGCTAGCTTTGTATAGTTCATC-3′.

Techniques: Western Blot, Expressing, Co-Culture Assay, Fluorescence, Incubation, Two Tailed Test